Integration of microfluidic chip with a microcontrol system for stabilization of the temperature for diagnosis of tuberculosis by loop mediated isothermal amplification

Abstract

This Master thesis was supported by TUBITAK with project code of 115R002. Aim of this project is to reproduce isolated DNA from patient's phelgm by LAMP method with stabilized temperature by microcontroller on microfluidic chip and in case od success, this prepared microchip is aimed to be commercilized. This project has 5 main progresses: design, production and characterization of microfluidic chip with heating element and reaction chamber where reaction is occured, circuit of microcontroller design to control temperature, characterization and integration, preparation of biological mixture to fill reaction reservoir and optimization, filling prepared biological solution to the reaction reservoir in prototype and optimization of prototype according to data obtained and determination of shelf life of the prototype. In this thesis not only for microfluidic chip but also for microcontroller: production, design, modelling and characterization were done also analysis of the data obtained was interpreted.