A multi-stage computational pipeline and <i>in vitro</i> validation for the discovery of small-molecule translation inhibitors targeting the bacterial ribosome

Abstract

A hierarchical VS pipeline to target the bacterial ribosome PTC and in vitro translation assays identified Mitoxantrone as a potent inhibitor of E. coli protein synthesis, and Plerixafor, Olcegepant, and Ziritaxestat as lead compounds.