The first identification of Tula orthohantavirus in forest dormice (Rodentia: Gliridae) from Iran

Abstract

Abstract Orthohantaviruses are a group of zoonotic pathogens that can cause severe and potentially fatal syndromes in humans. Although orthohantaviruses have been documented in Iran, little is known about their potential reservoirs in the country. The present study constitutes the first attempt to detect orthohantavirus infections in rodents in Iran. Sampling was performed in the East Azerbaijan province, during October 2017 and June 2018. To detect orthohantavirus infection, we performed a Pan-hantavirus nested reverse transcription-polymerase chain reaction targeting the large segment of the viral genome using consensus primers on lung tissue samples. We identified Tula orthohantavirus for the first time in Iran and from Dryomys nitedula sensu lato (s.l.) (Gliridae), a non-muroid host. There are two possible interpretations for this finding: an interspecies spillover or the presence of a broader primary host/reservoir list. However, at present, we do not advocate for either interpretation, but instead, argue for further accumulation of relevant data. Phylogenetic analysis revealed a close relationship between the Tula orthohantavirus identified in this study and those detected in Turkey. Nevertheless, comprehensive field studies, as well as full-length sequence analyses, are required to gain a better understanding of the potential reservoirs, ecology, and epidemiological cycle of the virus in Iran.