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BMP-2 immobilized PCL/P3ANA nanofibers for bone tissue engineering

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dc.contributor.authorGuler, Zeliha
dc.contributor.authorSarac, A. Sezai
dc.date.accessioned2026-01-25T05:21:49Z
dc.date.issued2015-11-01
dc.description.abstractPoly (e-caprolactone)/poly (m-anthranilic acid) (PCL/P3ANA) nanofibers were fabricated by electrospinning. Nanofibers were biofuctionalized with Bone morphogenic protein-2 (BMP-2) with 1-ethyl-3-(dimethyl-aminopropyl) carbodiimide hydrochloride (EDC)/N-hydroxyl succinimide (NHS) surface activation of carboxyl groups (-COOH) on PCL/P3ANA scaffold. The structure of nanofibers was investigated by FTIR-ATR spectroscopy. The amount of bound protein was determined by BCA protein assay. In vitro cell culture studies were conducted by using human osteocarsinoma cell line (SAOS-2) and protein immobilized PCL/P3ANA nanofibers as tissue engineering scaffolds. Cell viability and proliferation assays were performed in order to understand the cell behaviour against PCL/P3ANA nanofibers. Fluorescence staining of F-actin was performed to observe the cell morphology. Alkaline phoshotase (ALP) activity of SAOS-2 cells were investigated. The results confirmed the successful immobilization of BMP-2 on the PCL/P3ANA nanofibers which can be used in bone tissue engineering.
dc.description.urihttps://doi.org/10.1109/ehb.2015.7391452
dc.description.urihttps://dx.doi.org/10.1109/ehb.2015.7391452
dc.identifier.doi10.1109/ehb.2015.7391452
dc.identifier.endpage4
dc.identifier.openairedoi_dedup___::6c8449b0b0e54cd35316387fa57e748b
dc.identifier.orcid0000-0001-7293-8151
dc.identifier.orcid0000-0001-7513-1740
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/11527/46940
dc.publisherIEEE
dc.relation.ispartof2015 E-Health and Bioengineering Conference (EHB)
dc.sdg.typeGoal 3: Good Health and Well-being
dc.titleBMP-2 immobilized PCL/P3ANA nanofibers for bone tissue engineering
dc.typeArticle
dspace.entity.typePublication

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