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A New Approach for in vitro Imaging of Breast Cancer Cells by Anti‐Metadherin Targeted PVA‐Pyrene

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dc.contributor.authorMedine, E. Ilker
dc.contributor.authorOdaci, Dilek
dc.contributor.authorGacal, Bahadir N.
dc.contributor.authorGacal, Burcin
dc.contributor.authorSakarya, Serhan
dc.contributor.authorUnak, Perihan
dc.contributor.authorTimur, Suna
dc.contributor.authorYagci, Yusuf
dc.date.accessioned2026-01-26T04:41:50Z
dc.date.issued2010-06-09
dc.description.abstractAbstractPoly(vinyl alcohol)‐pyrene‐anti‐metadherin (PVA‐Py‐(Anti‐MTDH)), a novel antibody based water soluble probe containing both fluorescent and target sites in the structure for in vitro imaging of breast cancer cells is reported here. Since breast cancer cells have an excess of MDTH protein expressed on the surface, a PVA‐Py prepared by “Click chemistry” approach is targeted by Anti‐MTDH antibody and applied to the MCF‐7 cell line. After characterization, the designed architecture was evaluated in terms of cell incorporation efficiency and compared with a non‐targeted structure (PVA‐Py). Atomic force microscopy (AFM) and fluorescence microscopy images of cells after incubation of the probe molecules were also obtained to monitor the interaction of the probes with the cancerous cells.magnified image
dc.description.urihttps://doi.org/10.1002/mabi.201000009
dc.description.urihttps://pubmed.ncbi.nlm.nih.gov/20336700
dc.description.urihttps://dx.doi.org/10.1002/mabi.201000009
dc.description.urihttps://hdl.handle.net/11454/27113
dc.description.urihttps://hdl.handle.net/11454/43737
dc.description.urihttps://aperta.ulakbim.gov.tr/record/24013
dc.identifier.doi10.1002/mabi.201000009
dc.identifier.eissn1616-5195
dc.identifier.endpage663
dc.identifier.issn1616-5187
dc.identifier.openairedoi_dedup___::e2de809657115a38030b121981d6ecf7
dc.identifier.orcid0000-0002-7954-1381
dc.identifier.orcid0000-0002-1981-7577
dc.identifier.startpage657
dc.identifier.urihttps://hdl.handle.net/11527/61104
dc.identifier.volume10
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofMacromolecular Bioscience
dc.rightsOPEN
dc.sdg.typeGoal 3: Good Health and Well-being
dc.subjectDiagnostic Imaging
dc.subjectImmunoconjugates
dc.subjectsynthesis
dc.subjectAntibodies, Neoplasm
dc.subjectFunctionalization of polymers
dc.subjectBreast Neoplasms
dc.subjectFluorescence
dc.subjectImaging
dc.subjectdrug delivery systems
dc.subjectSynthesis
dc.subjectCell Line, Tumor
dc.subjectHumans
dc.subjectfunctionalization of polymers
dc.subjectFluorescent Dyes
dc.subjectPyrenes
dc.subjectimaging
dc.subjectMembrane Proteins
dc.subjectRNA-Binding Proteins
dc.subjectDrug delivery systems
dc.subjectNeoplasm Proteins
dc.subjectPolyvinyl Alcohol
dc.subjectFemale
dc.subjectfluorescence
dc.subjectCell Adhesion Molecules
dc.titleA New Approach for in vitro Imaging of Breast Cancer Cells by Anti‐Metadherin Targeted PVA‐Pyrene
dc.typeArticle
dspace.entity.typePublication

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