Determination of eugenol in plants and pharmaceutical form by HPLC with amperometric detection at graphene-modified carbon paste electrode

Abstract

Eugenol is an important natural compound derived from various plants, mainly in clove, and used for healthy benefits, cosmetics and as flavoring agent. A high-performance liquid chromatography (HPLC) coupled to electrochemical detection method was developed for determination eugenol in several methanol extract of plants and pastille. The separation was carried out on a RP-HPLC C18 column, isocratically in 1% acetic acid–acetonitrile–methanol (43:17:40 v/v) mobile phase system with amperometric detection at carbon paste electrode modified with graphene, versus Ag/AgCl at 0.9 V. The range of eugenol concentration varied from 0.16 to 16.42 µg/mL with good coefficient of determination (r 2 = 0.9994). The limit of detection and limit of quantification values of eugenol were 0.052 and 0.157 µg/mL, respectively.