Spectrofluorometric Determination of Hydrogen Peroxide

Abstract

A Spectrofluorometric method for microdetermination of H2O2 has been developed. The method is based on the oxidation of hydrogen peroxide with ceric ion in acid solution and measurement of the fluorescence during titration of the Ce(III) ions produced. The fluorescent species have excitation and emission maxima at 260 and 360 nm, respectively. The detection limit of measurement by this method was 0.1 ppm hydrogen peroxide.