Antioxidant, anticholinesterase and antimicrobial constituents from the essential oil and ethanol extract of Salvia potentillifolia

Abstract

The essential oil of Salvia potentillifolia was analysed by GC and GC–MS. Totally, 123 components were detected in both hydrodistilled and steam-distilled oils, a- and b-pinenes being major compounds. The antioxidant activities were determined by using complementary tests, namely, DPPH radical-scavenging, b-carotene-linoleic acid and reducing power assays. The ethanol extract also showed better activity (IC50 = 69.4 ± 0.99 lg/ml) than that of BHT in the DPPH system, and showed great lipid peroxidation inhibition in the b-carotene-linoleic acid system (IC50 = 30.4 ± 0.50 lg/ml). The essential oil showed meaningful butyrylcholinesterase activity (65.7 ± 0.21%), and a-pinene showed high acetylcholinesterase inhibitory activity (IC50 = 86.2 ± 0.96 lM) while b-pinene was inactive. Antimicrobial activity was also investigated on several microorganisms, and the essential oil showed high activity against Bacillus subtilis and B. cereus. It also exhibited remarkable anticandidal activity against Candida albicans and C. tropicalis with MIC values of 18.5 and 15.5 lg/ml, respectively, while a- and b-pinenes showed moderate activity.