Publication:
Immobilization of invertase in conducting thiophene-capped poly(methylmethacrylate)/polypyrrole matrices

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dc.contributor.authorAlkan, S.
dc.contributor.authorToppare, L.
dc.contributor.authorYagci, Y.
dc.contributor.authorHepuzer, Y.
dc.date.accessioned2026-01-26T04:04:44Z
dc.date.issued1999-01-01
dc.description.abstractImmobilization of invertase in thiophene-capped poly(methylmethacrylate)/polypyrrole matrices was achieved by constant potential electrolysis using different supporting electrolytes. Optimum reaction conditions such as substrate concentration, temperature, and pH for the enzyme electrodes were determined. The temperature and pH were found to be 60 degrees C and 4.8, respectively. The effect of supporting electrolyte on the enzyme activity revealed that SDS was the best in the immobilization procedure. Michaelis-Menten constant and the maximum reaction rate in PMMA/PPy matrices were of the order of that of pristine polypyrrole. However, in terms of repeated use, the copolymer matrices were superior to polypyrrole.
dc.description.urihttps://doi.org/10.1163/156856299x00036
dc.description.urihttps://pubmed.ncbi.nlm.nih.gov/10673018
dc.description.urihttps://dx.doi.org/10.1163/156856299x00036
dc.identifier.doi10.1163/156856299x00036
dc.identifier.eissn1568-5624
dc.identifier.endpage1235
dc.identifier.issn0920-5063
dc.identifier.openairedoi_dedup___::da895f53de1a7b1cdcb854237422f2b6
dc.identifier.orcid0000-0002-7966-6352
dc.identifier.orcid0000-0003-4523-345x
dc.identifier.startpage1223
dc.identifier.urihttps://hdl.handle.net/11527/60067
dc.identifier.volume10
dc.language.isoeng
dc.publisherInforma UK Limited
dc.relation.ispartofJournal of Biomaterials Science, Polymer Edition
dc.subjectGlycoside Hydrolases
dc.subjectbeta-Fructofuranosidase
dc.subjectPolymers
dc.subjectSurface Properties
dc.subjectElectric Conductivity
dc.subjectTemperature
dc.subjectThiophenes
dc.subjectHydrogen-Ion Concentration
dc.subjectEnzymes, Immobilized
dc.subjectElectrolysis
dc.subjectKinetics
dc.subjectMicroscopy, Electron, Scanning
dc.subjectPolymethyl Methacrylate
dc.subjectPyrroles
dc.titleImmobilization of invertase in conducting thiophene-capped poly(methylmethacrylate)/polypyrrole matrices
dc.typeArticle
dspace.entity.typePublication

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