Assessing the effect of selenium on cyclin D1 level and nuclear factor kappa b activity in NIH/3T3 fibroblast cells at 2100 MHz electromagnetic field exposure

Abstract

Abstract Purpose: Although there are various studies on the biological effects of the 2100 MHz electromagnetic field (EMF), which is among the mobile phone frequencies, there are conflicting results in the literature on this subject. Materials and Methods: The present study was designed to assess the effect of 200 nM selenium (Se) on cell viability% [trypan blue], cell cycle biomarker [cyclin D1] and the transcription factor [nuclear factor kappa b (NF-κB)] in NIH/3T3 fibroblast cells exposed to 2100 MHz mobile phone frequency. Cells were divided to following groups: Control, sham control, 2100 MHz EMF, and 200 nM Se + 2100 MHz EMF for 2 h. And we measurement cell viability%, cyclin D1 level and NF-κB activity. Results and Conclusions: While of cell viability% decreased in 2100 MHz EMF group compared to control group, cell viability% increased in 200 nM Se + 2100 MHz EMF group compared to 2100 MHz EMF. It was also shown that while cyclin D1 level and NF-κB activity increased significantly in the 2100 MHz EMF group compared to the control group, the same parameters decreased in the 200 nM Se + 2100 MHz EMF group compared to the 2100 MHz EMF group. Although future studies will be required to investigate the biological effects of EMF emitted by mobile phones, our results provide insight into the molecular mechanisms underlying selenium's protective function and efficacy against 2100 MHz EMF exposure.