Gluconacetobacter hansenii p2a ile gerçekleştirilen bakteriyel selüloz üretiminin kinetik modellenmesi

Abstract

This study involved the expression of the kinetics of bacterial cellulose production by Gluconacetobacter hansenii P2A (KUEN 1606); a strain which was originally isolated from a rotten plum. Bacterial cellulose production was realized in Hestrin Schramm medium under static and agitated cultivation conditions. The experimental data were fitted to relevant kinetic models for cell growth and cellulose production and model parameters thereof were calculated accordingly. Results proved that the growth of Gluconacetobacter hansenii P2A was best described by Monod equation for both stationary and agitated culture conditions (R21). Monod parameters max and Ks were identified as 0.081 h-1, 45.88 g.l-1 and 0.224 h-1, 22.85 g.l-1 for static and agitated conditions, respectively. For the description of product formation kinetics, Leudeking Piret model was selected according to the relatively high regression coefficients of 0.96 and 0.98, for stationary and agitated culture conditions, respectively. These constructed models of high accuracy are convenient to form the basis for the design of pilot and industrial scale production of bacterial cellulose by Gluconacetobacter hansenii P2A (KUEN 1606)