Poly (ε-caprolactone) Synthesis By A Novel Enzymatic Catalyst: Candida Antarctica Lipase B (CALB) Immobilized On A Modified Silica-based Material By Physical Adsorption

Abstract

In the present study, ring opening polymerization of e -caprolactone was performed by a novel enzymatic catalyst, Candida antarctica lipase B (CALB) immobilized on a modified silica-based material by physical adsorption. Molecular weight distributions and chain structures were compared by using gel permeation chromatography (GPC) and hydrogen nuclear magnetic resonance ( 1 H NMR) analysis, respectively. In addition, for the determination of thermal properties, thermal gravimetric analysis (TGA) and differential scanning calorimetry (DSC) were performed. Scanning electron microscopy (SEM) was applied to observe the surface structure of the polymer. Effects of temperature, reaction time, and enzyme concentration on molecular weight of poly (e